Block Copolymer Nanomicelle-Encapsulated Curcumin Attenuates Cerebral Ischemia Injury and Affects Stem Cell Marker Expression by Inhibiting lncRNA GAS5

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Introduction
Stroke has become the most common cause of death in China [1] of which IS accounts for the vast majority, reaching 70% to 80% [2]. At present, the effective treatment methods for IS are mainly to open arteries and restore blood flow as soon as possible [3,4] including intravenous thrombolysis and mechanical clot thrombectomy. However, due to the limitation of effective treatment time and other conditions, the vast majority of IS patients fail to receive these treatments. At the same time, the early vascular opening is not completely equivalent to a good curative effect, and the current statistical effective rate in the world is less than 50%. It is still of great importance to search for protective strategies for cerebral ischemia injury after IS.
Curcumin is a small molecule polyphenolic compound extracted from traditional Chinese medicine turmeric [5,6]. It has anti-inflammatory, antioxidative stress, and antiapoptotic pharmacological effects [7]. It has an obvious protective effect on ischemic brain tissue [8]. However, due to its low water solubility, low uptake rate in vivo, and high blood clearance rate, curcumin has low bioavailability and cannot be used in clinical practice. Meanwhile, its neuroprotective mechanism of action has not been fully elucidated. Hydrophobic polycaprolactone (PCL) and hydrophilic polyethylene glycol (mPEG) copolymer carriers have low toxicity, high stability, and biodegradability. The natural polymer polyglutamic acid (PLG) can form an amphiphilic polymer with hydrophilic substances and can physically encapsulate hydrophobic drugs by using physical forces such as electrostatic adsorption or pi-pi stacking. Therefore, PLG-mPEG-PCL triblock polymer nanomicelles can be used as carrier materials to achieve the solubility and controlled release of curcumin.
Long noncoding RNAs (lncRNAs) are noncoding RNAs. The length of these noncoding RNA was found more than 200 nt. They have powerful regulatory roles including not only transcriptional regulation but also posttranscriptional regulation and chromatin modification [9]. In addition, lncRNAs can also regulate a variety of biological processes. Their abnormal expression may trigger various neurodegenerative diseases, such as parkinsonism [10], multiple sclerosis (MS) [11], and IS [12,13]. It has been reported that lncRNAs, specifically growth arrest-specific transcript 5 (GAS5), affect neuronal apoptosis and function in IS [12]. Moreover, highly expressed GAS5 is not only associated with an increased risk of IS but also promotes neuronal apoptosis after cerebral infarction [14,15]. GAS5 plays a critical role in various human cancers [16]. However, the effect of curcumin on lncRNA GAS5 is rarely reported. Therefore, the regulation of curcumin on lncRNA GAS5 is also included in this study. This research also explores whether curcumin can affect ischemic injury through lncRNA GAS5.
Despite advances in innovative treatment strategies, stroke remains one of the leading causes of death and disability worldwide. Due to significant therapeutic effects on patients with stroke, stem cell therapy has received extensive attention. Various types of cells (bone marrow mononuclear cells, bone marrow/adipose-derived stem/stromal cells, umbilical cord blood cells, neural stem cells, and olfactory ensheathing cells) have been shown to improve neurological function in animal models of stroke [17]. Therefore, in this study, we also tested some stem cell markers to explore the possible regulatory relationship among curcumin, lncRNA GAS5, and stem cell markers which have never been reported before. In this study, rats' neurons were first extracted and treated with glucose and oxygen deprivation. Multiple interference groups were set up, and we explored the regulatory relationship between curcumin and lncRNA GAS5 at the same time; their effects on inflammation-related factors, cell activity, and stem cell markers were also explored. This was further verified in animals. Curcumin and lncRNA GAS5 effects on cerebral ischemic cell damaging by affecting stem cell differentiation have been revealed in this study.

Preparation of Curcumin Nanomicelles Encapsulated by
Triblock Copolymer. mPEG was grafted on the PLG segment through esterification reaction, a certain amount of ε-CL and mPEG was put into a dry polymerization tube treated with silicon chemical treatment, and we added catalyst Sn(Oct)2 and kept stirring it gently at 130°C for 6 hours. After vacuum degassing, cooling, purification, and drying, it was stored in a closed container for later use. Copolymer characterization tests were then performed. Hydrogen nuclear magnetic resonance (1H NMR) was first characterized by NMR (nuclear magnetic resonance) and relative molecular mass. The polymer thermal properties were then investigated with a NETZSCH DSC 204F1 instrument. The samples were then pressed with potassium bromide (KBr) and tested for infrared spectra on an FT-IR Spectrum BX infrared spectrometer. Dynamic light scattering (DLS) was performed by a Wyatt QELS instrument (Wyatt Technology) with a vertically polarized He-Ne laser (at 90°C) for detecting particle size and potential. Finally, fluorescence probe spectroscopy was used to detect the critical micelle concentration (CMC). Pyrene was used as a probe. Fluorescent probe technology was used to detect the ratio of CMC value. It was also used for the detection of the CMC value of mixed micelles of three groups (amphiphilic polymers with different hydrophilic block ratios).
An appropriate amount of curcumin and PLG-mPEG-PCL dissolved in acetone was used as a lipid phase, and an appropriate amount of poloxamer was dissolved in ultrapure water to form an aqueous phase. The lipid and aqueous phases were installed in separate tubes, and a syringe pump was set to inject the lipid and aqueous phases into the microchannels at a specific flow rate. The effluent was collected and centrifuged at low temperature and high speed for 30 min. The precipitate was ultrasonically dispersed in an appropriate amount of distilled water and then was freezedried for 24 h to obtain powdered curcumin nanoparticles which were stored at 4°C in the dark.

Identification of Triblock Copolymer-Encapsulated
Curcumin Nanomicelles. Transmission electron microscopy (TEM) was used to observe the morphologies of blank micelles and drug-loaded micelles. Encapsulation efficiency (EE) and drug loading (LC) were determined. The drug release of the drug-loaded micelles was measured under physiological conditions, the concentration of curcumin was measured by UV light, and the cumulative drug release rate was calculated to obtain the in vitro release curve. A certain concentration of curcumin-loaded micelles was added to the VSMCs (vascular smooth muscle cells); cell culture medium and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) analyses were performed to calculate the relative cell viability. Subsequently, in vivo pharmacokinetic assays were performed. The drugcontaining rats' plasma samples, blank rats' plasma samples, and curcumin methanol control solution were taken, and the plasma curcumin content was analyzed by HPLC (high-performance liquid chromatography), and the relevant pharmacokinetic parameters were obtained by calculation. The same batch of polymer micelle solution/lyophilized powder formulation was left standing at different temperatures for a long time (4°C and normal temperature). Samples were taken at regular intervals to measure drug encapsulation 2 Stem Cells International efficiency, particle size, and particle size distribution. The above data changes were used to analyze its long-term stability.

Culture and Identify Primary Rat Hippocampal Neurons.
SD (Sprague-Dawley) rats were purchased and bred. Rat hippocampal neurons were isolated from SD rats within 24 h of the newborn and placed in a 37°C CO 2 incubator which was kept being cultured for 7 days with medium changes every three days. Neurons were identified in vitro using neuron-specific diluteolase (NSE) for subsequent experiments.

Oxygen-Glucose Deprivation (OGD) Model Establishment.
The rat hippocampal neurons were placed in the MIC-101 (molecular incubator chamber-101) anaerobic culture system. The hypoxic chamber was connected to nitrogen, and the conditions were 95% N and 25% CO 2 . After being placed in a constant temperature incubator at 37°C for 3 hours, the sugar-free DMEM was replaced with a neuron medium (98% neurobasal medium+2% B27), and the rat hippocampal neurons were replaced in a CO 2 incubator under normoxic concentration at 37°C, 5% for 6-24 h.

Construct lncRNA GAS5 Overexpression/Knockout
Vector by CRISPR/Cas9 Technology. We designed the oligo DNA sequence of CRISPR/Cas9. sgRNA plasmid was constructed, transformed, and inoculated, and clones were picked. Plasmid DNA was extracted, and single-stranded oligonucleotides against the target gene locus were synthesized. The plasmid was electrotransfected into cells. 24 hours after transfection, the transfection efficiency was detected by qRT-PCR for subsequent experiments. The cells were divided into different groups: the control group (control), the model group (copolymer), the model+curcumin nanomicelle group (CC), the model+lncRNA GAS5 interference group (copoly-mer+GAS5 inhibitor), the model+lncRNA GAS5 overexpression group (copolymer+GAS5-plasmid), the model +curcumin nanomicelles+lncRNA GAS5 overexpression group (CC+GAS5-plasmid), and the model+curcumin nano-micelles+lncRNA GAS5 interference group (CC+GAS5 inhibitor). Among them, curcumin nanomicelles (concentration of 50 μM) were added to the medium of the intervention group supplemented with curcumin nanomicelles.
2.6. The CCK8 Assay. The survival rate of cortical neurons was measured by the CCK8 method for 24 h after oxygen and glucose deprivation. Cells were seeded according to the instructions. Fresh medium (100 μL) containing CCK8 solution (10 μL) was added to each well of the 96-well plate in the dark and placed in an incubator to incubate in the dark for 2 h. After that, the cell proliferation rate was detected at 450 nm using a microplate reader.

ELISA.
The cytokine levels such as IL-1β, IL-6, and TNF-α in cells, plasma, and brain tissue of each group were detected by ELISA (Invitrogen™ Cat. No. CNB0011). The specific operation method was referred to in the kit instructions.

Preparation of Ischemic Stroke (Suture Method MACO)
Model. The model groups were fed the corresponding drugs by gavage for 14 days before modeling. To analyze the impairment of neurons in SD rats, MACO model was established [19]. Two groups named as operation and sham were used in the model. In the operation group, the right middle cerebral artery was blocked by a suture to achieve focal cerebral ischemia, and the wound was sutured. Rats were kept in a single cage after the operation, and the suture was gently withdrawn from the ICA 2 hours later to realize reperfusion. In the sham-operated group, only the right carotid artery was isolated, no suture was inserted, and no neurological damage was observed after the operation.
The success criteria of the model were wrist-elbow flexion and shoulder internal rotation of the right forelimb and inability to stretch forward grasp or tilt to the opposite side. Rats with no neurological impairment and subarachnoid hemorrhage were excluded.
Neurobehavioral scores were used to judge the animal models. The Zea-longa scoring method was used. 0-4 score was given according to the level of neural damage. Scores between 1 and 3 indicated successful modeling, and rats with scores of 0 and 4 were excluded. The 0 score indicated the no impairment in neural functions, while 4 score represented the acute damage in the neural functions.
2.13. Statistical Methods. The experimental data of this study were statistically analyzed by using GraphPad software (https://www.graphstats.net/graphpad-prism). Differences between the two groups (control and experimental or model) were calculated using an unpaired t-test. When P value < 0.05, this means the difference is statistically significant.

Results
To investigate the protective mechanism of cerebral ischemia injury after IS. Cerebral ischemia injury models in vivo MACO rat and in vitro (oxygen-glucose deprivation cell model) were constructed. The expressions of lncRNA in neuronal cells, brain tissue, and plasma were detected by RT-PCR. The expression of the protein in neuronal cells, brain tissue, and plasma was determined by ELISA and western blot. Cell activity was detected by the CCK-8 assay. TUNEL assay determines cell apoptosis. Curcumin can inhibit the expression of lncRNA GAS5 in the rats' neuronal cells and brain tissue. In neuronal cells, plasma, and brain tissue, curcumin and the low-expressed lncRNA GAS5 can inhibit the expression of IL-1β, TNF-α, IL-6, Sox2, Nanog, and Oct4.
The spherical-shaped nanomicelles were observed by TEM. The mean diameter of the nanomicelles was less than 53 nm, while calculated value for zeta potential was +42.1. Change in the size of nanomicelles evaluated the encapsula-tion of curcumin nanomicelle while the encapsulation process was confirmed by various assays, which showed the decreased expression of lncRNA GAS5.

Triblock Copolymer-Encapsulated Curcumin Nanomicelles
Inhibit lncRNA GAS5 Expression. To verify the effects of lncRNA GAS5 inhibitor and lncRNA GAS5 plasmids on lncRNA GAS5 expression, we first verified their transfection efficiency in cells. The results demonstrated that the lncRNA GAS5 inhibitor could significantly inhibit the expression of lncRNA GAS5 (Figure 1(a)), while the lncRNA GAS5 plasmid could significantly upregulate the expression of lncRNA GAS5 (Figure 1(b)). Subsequently, we set up different interference groups to explore the effect of triblock copolymerencapsulated curcumin nanomicelles on the expression of lncRNA GAS5. The results showed that curcumin nanomicelles encapsulated by triblock copolymers could significantly inhibit the expression of lncRNA GAS5 (Figure 1(c)).

Triblock Copolymer-Encapsulated Curcumin Nanomicelles
Enhance Cell Viability and Inhibit the Expression of IL-1β, TNF-α, and IL-6 via lncRNA GAS5. To explore the effect of triblock copolymer-encapsulated curcumin on cell viability, we conducted cell viability experiments. The results showed that both triblock copolymer-encapsulated curcumin nanomicelles and low-expressed lncRNA GAS5 could enhance cell viability. But in the triblock copolymer-encapsulated curcumin nanomicelles and the lncRNA GAS5 interference group (CC+GAS5-plasmid), the enhancement effect disappeared (Figure 2(a)). It can be inferred that the curcumin nanomicelles encapsulated by triblock copolymers enhance cell activity by inhibiting the expression of lncRNA GAS5. Subsequently, to explore the effect of triblock copolymerencapsulated curcumin on ischemic injury, we interfered with the cells after hypoxia deprivation and detected the expressions of IL-1β, TNF-α, and IL-6 by the ELISA experiment. The results showed that triblock copolymerencapsulated curcumin nanomicelles and low-expressed lncRNA GAS5 significantly inhibited the expression of IL-1β (Figure 2(b)), TNF-α (Figure 2(c)), and IL-6 ( Figure 2(d)). However, in the triblock copolymer-encapsulated curcumin nanomicelles and lncRNA GAS5 interference group (CC +GAS5-plasmid), this inhibitory effect disappeared. This proved that the block copolymer-encapsulated curcumin nanomicelles inhibited the expression of IL-1β, TNF-α, and IL-6 by inhibiting the lncRNA GAS5. The result of the TUNEL assay showed that both triblock copolymer-encapsulated curcumin nanomicelles and low-expressed lncRNA GAS5 could decrease cell apoptosis. But in the triblock copolymerencapsulated curcumin nanomicelles and the lncRNA GAS5 interference group (CC+GAS5-plasmid), the effect disappeared (Figure 2(e)).

Triblock Copolymer-Encapsulated Curcumin Nanomicelles
Inhibit the Expression of Stem Cell Markers by Inhibiting lncRNA GAS5. To explore the effect of triblock copolymerencapsulated curcumin nanomicelles and lncRNA GAS5 on stem cells, we detected stem cell markers. The results showed that curcumin nanomicelles encapsulated by 4 Stem Cells International triblock copolymers can inhibit the expression of stem cell markers Sox2 (Figure 3(a)), Nanog (Figure 3(b)), and Oct4 (Figure 3(c)) and inhibit the expression of lncRNA GAS5 achieving the same effect. According to the previous results, triblock copolymer-encapsulated curcumin nanomicelles can downregulate the expression of lncRNA GAS5. Therefore, we speculate that curcumin nanomicelles encapsulated by triblock copolymers can regulate stem cell markers by inhibiting lncRNA GAS5. The expression of Sox2 (Figure 3(a)), Nanog (Figure 3(b)), and Oct4 (Figure 3(c)) in the CC+GAS5plasmid group and the control group was not significantly different, which also confirmed this.

The Effect of Triblock Copolymer-Encapsulated Curcumin
Nanomicelles on lncRNA GAS5 in Animals and Its Subsequent Influence. To verify whether the regulation of lncRNA GAS5 by triblock copolymer-encapsulated curcumin nanomicelles and its subsequent effects in animals were consistent with the previous cell experiments, we conducted follow-up animal experiments. Animal models were judged using behavioral scores, and mice with scores 1-4 were selected for follow-up studies (Figure 4(a)). The transfection effect in animals of the interference group was verified, the expression of lncRNA GAS5 in different interference groups was detected, and the results were consistent with expectations (Figure 4(b)). Plasma and brain tissue were extracted for detection. Plasma results showed that curcumin nanomicelles encapsulated by triblock copolymer and low-expressed lncRNA GAS5 could significantly inhibit the expression of cytokines IL-1β, TNF-α, and IL-6 ( Figures 5(a)-5(c)).
After upregulating the expression of lncRNA GAS5, this inhibitory effect was also alleviated. Similar results were obtained after testing brain tissue. In brain tissue, curcumin nanomicelles encapsulated by triblock copolymer and low-expressed lncRNA GAS5 can also significantly inhibit the expression of cytokines IL-1β, TNF-α, and IL-6 ( Figures 5(d)-5(f)), while this inhibitory effect was also alleviated after upregulating the expression of lncRNA GAS5.
Subsequently, we detected stem cell markers in plasma and brain tissue. Plasma results showed that curcumin nanomicelles encapsulated by triblock copolymers could inhibit the expression of stem cell markers Sox2, Nanog, and Oct4 (Figures 6(a)-6(c)). The low-expressed lncRNA GAS5 can inhibit the expression of Sox2, Nanog, and Oct4, while the high-expressed lncRNA GAS5 can promote the expression of Sox2, Nanog, and Oct4 (Figures 6(a)-6(c)). The expression of curcumin nanomicelles encapsulated by triblock copolymers and overexpressed lncRNA GAS5, Sox2, Nanog, and Oct4 had no significant difference from the control group. Brain tissue results also showed that curcumin nanomicelles encapsulated by triblock copolymers could inhibit the expression of stem cell markers Sox2, Nanog, and Oct4 (Figures 6(d)-6(f)). Similar results can be achieved by inhibiting the expression of lncRNA GAS5. Similarly, curcumin nanomicelles encapsulated by triblock copolymers and overexpressed lncRNAs GAS5, Sox2, Nanog, and Oct4 showed no significant difference from the control group (Figures 6(d)-6(f)). The above results were consistent with the results of cell experiments, indicating that curcumin nanomicelles encapsulated by triblock copolymers can

Discussion
Through experiments in rats' neuronal cells, we found that curcumin can inhibit the expression of lncRNA GAS5. In addition, curcumin can also increase the cell activity of neuronal cells after the glucose-oxygen deprivation experiment, and inhibiting the expression of lncRNA GAS5 can also achieve the result of enhancing cell activity. This was similar to previous reports that curcumin and lncRNA GAS5 can promote cell survival [20][21][22]. Expression levels of GAS5 in various tumors have been studied [16]. However, adding curcumin while overexpressing the lncRNA GAS5 made this effect disappear. This indicated that curcumin enhances cell activity by inhibiting lncRNA GAS5.
Ischemic stroke can be caused by a variety of factors, such as cardiogenic embolism, occlusion of small cerebral vessels, and atherosclerosis affecting cerebral circulation. There is increasing evidence that the immune system plays   (e) TUNEL assay to detect cell apoptosis ( * P < 0:05, * * P < 0:01, and * * * P < 0:001; ns: not significant). 6 Stem Cells International a complex role in the pathophysiological changes that occur after cerebral ischemic injury. Following ischemic brain injury, we can observe the ensuing neuroinflammation causing additional damage and triggering cell death [23][24][25][26].